Yes, you should place order first in our website before sending samples to us.
Otherwise, we will NOT perform the experiment.
Print the order copy and send along with the samples to track your order.
You may place an order of require services from our on-line ordering system
using following link http://yaazhxenomics.in
Yes, We recommend you to use the online system all the time. It is much
more convenient and easy because you will be able to order online, get the
status and download / retrieve the results online.
No need of separate order for each sample type. Same order you can select
different type of services using “Continue to shopping” option according to
its sample type.
No, there is no such minimum number or maximum number of sequencing
We store your DNA & Primers for one month. If you want to sequence among
them, Place on additional order & Mention the previous order details.
Before placing the additional order please conform with us.
Yes, Microbial samples in agar plate or tube or Glycerol stock (1:1 ratio)
Yes. We do synthesize a primer.
We offers the oligonucleotide DNA synthesis service with the latest cutting-
edge technology and the state-of-the-art facility.
For normal microbial samples you can send it in Room temperature. If it is
PCR product we recommend to send it with one cool pack. Dry Ice is NOT
required. According to your sample requirement send it with dry ice. Usually
for RNA Samples Dry Ice shipment is recommended.
Yes, If more samples are with you we can come and collect the samples
directly in the following cities Madurai, Coimbatore, Chennai, Vellore, Mumbai,
Pune, Nagpur, Nashik, Kolkata, Vizagapatnam, Vijayawada, & Bangalore.
100% advance Payment via online using Debit card, Credit card, Net banking
Offline payment only allowed for Govt. institute / Specific Customers.
You can send DD/Cheque along with the samples in Favour of “Yaazh
For cash payment :
Name : Yaazh Xenomics
Account number : 01232000012649
Bank : HDFC bank,
Branch : West veli street, Madurai
RTGS/NEFT IFSC: HDFC0000123
Account Type : Current Account.
Please contact our online support Team or mail to firstname.lastname@example.org &
After completing sequencing order form you can choose your payment option
& Make payment.
Yes, We have various discount options for Large volume customers. Contact
email@example.com / firstname.lastname@example.org
We are having team of scientist they will provide support for your technical
You can mail your queries to email@example.com /
For PCR Product Sequencing: 2-4 working days from the date of sample
It may take 1 or 2 more working days in case of primer synthesis or
purification or any other additional work.
16s rRNA / 18s rRNA Sequencing: 5 – 7 working days from the date of
Customized Gene study: 7- 10 working days from the date of sample arrival
Next Generation Sequencing: Normally 3 to 6 weeks (Depends up on the
project and Gene size time may vary)
Yes, we have more than 30 Universal Primers Which we provide free of cost
for PCR & sequencing.
We recommend the primers to be sent in separate tubes with template DNA
so that we can adjust the amount of primer.
Concentration of plasmid / PCR product
Minimum 20ul of 100ng/ul(plasmids, unpurified PCR products) or
50ng/ul(purified PCR products) are required for a couple of reactions.
DNA Template Requirements
1) Purity: Gel or column purifed
2) Concentration: a) PCR products (>50ng/µl),b)Plasmids (100-150ng/µl),
c)Genomic DNA (> 500ng/µl)
3) Volume: At least 10 µl in distilled water
Concentration of Primer
20ul with concentration of 10pmole can cover 5 reactions. Please increase
10pmole each time the number of reactions is increasing.
We recommend the quality of HPLC or PAGE purified primers for sequencing
reactions, but OPC is o.k as long as it can guarantee the equivalent quality.
About 17-25 mer, 40~60% of GC content and less than 4bp of dimer formation
or hair-pin formation are recommended to guarantee high end stability. Tm is
required about 50~60°C, because annealing temperature is fixed at 50°C.
Primer concentration should be known before sequencing reactions.
1) Design: At least 18-20 bp long with a Tm > 45C, G-C content 30-80%
2) Purity: Desalted
3) Concentration: 10 µM
4) Volume: At least 5 µl in distilled water
1,500bp or longer sequence is guaranteed for domain bacteria
We do 18s rRNA / ITS region sequencing and we can find out the similarity of
customer's microorganism with microorganism in NCBI & with other latest
bioinformatics software tools.
Sequence results we will provide in different formats
*.ab1 – Sequencing instrument generated original file (Sequence details with
*.pdf – Same system file result (Result with chromatogram)
*.txt – Sequence details in text format (Without Chromatogram)
Along with Sequence result we will provide quality score file and
bioinformatics analysis files including phylogeny tree.
1.Contig sequence (Forward and Reverse sequence assembled data) in
details (Image &txt format).
2.Blastn & Blastx Results for Contig sequence & Individual sequence.
Yes, if you provide the details of the gene, PCR conditions and Primer details
then we do that sample process.
No, we do not provide PCR samples after sequencing. We store it for 1
month. If any other analysis needs to be done with in that period we can do it.
We use Applied Biosystem instrument – ABI PRISM 3730XL as well as
ABI3700. (96 Capillary Type)
MJ Research PTC-225 Peltier Thermal Cycler, & Biorad Thermal Cycler
ABI PRISM® BigDyeTM Terminator Cycle Sequencing Kits
InstaGene Matrix(Bio-Rad, USA) / Qiagen DNA Extraction Kits
We read approximately ~900bp per reaction, and 700bp of them has Phred 20
or higher quality.
The results are stored in our online portal for three months, after that results
will be discarded. We recommend you to download your results in your Hard
disk or other secure location as soon as they become available in the website.
Our AB1 files can be opened by any conventional software tools such as
Chromas, Finch TV, BioEdit, Vector NTI, and other so many applications.
For those customers not having these softwares, we provide Chromatogram in
PDF files as well.
Install any one of the following software tools to unzip. (winzip, 7zip, winrar)
Old No.3d/4, New no.15/1,
Palli arasan street, Anna Nagar East,
Chennai -600 102.
We send arrival notice on the same day of receiving the samples. If you
haven't received our arrival notice for one week, please contact us
immediately with the your courier tracking number. Probably there would be a
problem in unidentified sender, orderer. Or Shipment delivery problem.
Below are some of the possibility for not delivering the result.
1) Failure of sample delivery : Please provide us with the tracking number of
2) Failure of sample processing : Due to some reasons(such as lid is open on
the way, Sample contamination or no DNA in the tube) the samples may not
be processed to save your cost.
3) Failure of Reaction
In the case 2 & 3, our staff may have contacted you via email.
If you didn’t receive this email even in Spam/Junk folder, please contact us