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FAQ

Yes, you should place order first in our website before sending samples to us. 

 

 

Otherwise, we will NOT perform the experiment. 

 

 

 

 

Print the order copy and send along with the samples to track your order.

 

 

You may place an order of require services from our on-line ordering system 

using following link http://yaazhxenomics.in

Yes, We recommend you to use the online system all the time. It is much

more convenient and easy because you will be able to order online, get the 

status and download / retrieve the results online.

No need of separate order for each sample type.  Same order you can select

different type of services using “Continue to shopping” option according to 

its sample type.

No, there is no such minimum number or maximum number of sequencing 

reactions.

We store your DNA & Primers for one month. If you want to sequence among 

them, Place on additional order & Mention the previous order details. 

Before placing the additional order please conform with us.

Yes, Microbial samples in agar plate or tube or Glycerol stock (1:1 ratio)

Yes. We do synthesize a primer. 

We offers the oligonucleotide DNA synthesis service with the latest cutting-

edge technology and the state-of-the-art facility.

For normal microbial samples you can send it in Room temperature. If it is 

PCR product we recommend to send it with one cool pack.  Dry Ice is NOT 

required. According to your sample requirement send it with dry ice. Usually 

for RNA Samples Dry Ice shipment is recommended.

Yes, If more samples are with you we can come and collect the samples

directly in the following cities Madurai, Coimbatore, Chennai, Vellore, Mumbai, 

Pune, Nagpur, Nashik, Kolkata, Vizagapatnam,  Vijayawada, & Bangalore.

100% advance Payment via online using Debit card, Credit card, Net banking

Offline payment only allowed for Govt. institute / Specific Customers. 

You can send DD/Cheque along with the samples in Favour of “Yaazh 

Xenomics”

For cash payment :

Name : Yaazh Xenomics 

Account number : 01232000012649

Bank : HDFC bank, 

Branch : West veli street, Madurai 

RTGS/NEFT IFSC: HDFC0000123 

Account Type : Current Account.

Please contact our online support Team or mail to payment@yaazhxenomics.in & 

info@yaazhxenomics.com

After completing sequencing order form you can choose your payment option 

& Make payment.

Yes, We have various discount options for Large volume customers. Contact

info@yaazhxenomics.com / enquiry@yaazhxenomics.com

We are having team of scientist they will provide support for your technical 

queries.

You can mail your queries to parthi@yaazhxenomics.com / 

info@yaazhxenomics.com

For PCR Product Sequencing: 2-4 working days from the date of sample 

arrival.

It may take 1 or 2 more working days in case of primer synthesis or 

purification or any other additional work.

16s rRNA / 18s rRNA Sequencing:  5 – 7 working days from the date of 

sample arrival

Customized Gene study: 7- 10 working days from the date of sample arrival

Next Generation Sequencing: Normally 3 to 6 weeks (Depends up on the 

project and Gene size time may vary)

Yes, we have more than 30 Universal Primers Which we provide free of cost

for PCR & sequencing.

We recommend the primers to be sent in separate tubes with template DNA

so that we can adjust the amount of primer.

Concentration of plasmid / PCR product

Minimum 20ul of 100ng/ul(plasmids, unpurified PCR products) or 

50ng/ul(purified PCR products) are required for a couple of reactions.

DNA Template Requirements    

1) Purity: Gel or column purifed                

2) Concentration: a) PCR products (>50ng/µl),b)Plasmids (100-150ng/µl), 

c)Genomic DNA (> 500ng/µl)

3) Volume: At least 10 µl in distilled water 

           

Concentration of Primer

20ul with concentration of 10pmole can cover 5 reactions. Please increase 

10pmole each time the number of reactions is increasing.

We recommend the quality of HPLC or PAGE purified primers for sequencing 

reactions, but OPC is o.k as long as it can guarantee the equivalent quality. 

About 17-25 mer, 40~60% of GC content and less than 4bp of dimer formation 

or hair-pin formation are recommended to guarantee high end stability. Tm is 

required about 50~60°C, because annealing temperature is fixed at 50°C. 

Primer concentration should be known before sequencing reactions.

Primer Requirements                                    

1) Design: At least 18-20 bp long with a Tm > 45C,  G-C content 30-80% 

(prefer 50-55%)      

2) Purity: Desalted           

3) Concentration: 10 µM               

4) Volume:  At least 5 µl in distilled water

1,500bp or longer sequence is guaranteed for domain bacteria

We do 18s rRNA / ITS region sequencing and we can find out the similarity of 

customer's microorganism with microorganism in NCBI & with other latest 

bioinformatics software tools.

Sequence results we will provide in different formats

*.ab1 – Sequencing instrument generated original file (Sequence details with 

chromatogram)

*.pdf – Same system file result (Result with chromatogram)

*.txt – Sequence details in text format (Without Chromatogram)

Along with Sequence result we will provide quality score file and 

bioinformatics analysis files including phylogeny tree.

1.Contig sequence (Forward and Reverse sequence assembled data) in 

details (Image &txt format).

2.Blastn & Blastx Results for Contig sequence & Individual sequence.

3.Phylogenetic

Yes, if you provide the details of the gene, PCR conditions and Primer details 

then we do that sample process.

No, we do not provide PCR samples after sequencing. We store it for 1

month. If any other analysis needs to be done with in that period we can do it.

We use Applied Biosystem instrument – ABI PRISM 3730XL as well as

ABI3700. (96 Capillary Type)

MJ Research PTC-225 Peltier Thermal Cycler,  & Biorad Thermal Cycler

ABI PRISM® BigDyeTM Terminator Cycle Sequencing Kits

InstaGene Matrix(Bio-Rad, USA) / Qiagen DNA Extraction Kits

We read approximately ~900bp per reaction, and 700bp of them has Phred 20

or higher quality.

The results are stored in our online portal for three months, after that results 

will be discarded. We recommend you to download your results in your Hard 

disk or other secure location as soon as they become available in the website.

Our AB1 files can be opened by any conventional software tools such as 

Chromas, Finch TV, BioEdit, Vector NTI, and other so many applications. 

For those customers not having these softwares, we provide Chromatogram in 

PDF files as well.

Install any one of the following software tools to unzip. (winzip, 7zip, winrar)

Mr.Sundar.

Yaazh Xenomics.

Old No.3d/4, New no.15/1,

Palli arasan street, Anna Nagar East,

Chennai -600 102.

Tamilnadu.

Ph:+91-98400 44239.

We send arrival notice on the same day of receiving the samples. If you

haven't received our arrival notice for one week, please contact us 

immediately with the your courier tracking number. Probably there would be a 

problem in unidentified sender, orderer. Or Shipment delivery problem.

Below are some of the possibility for not delivering the result.

1) Failure of sample delivery : Please provide us with the tracking number of 

your samples. 

2) Failure of sample processing : Due to some reasons(such as lid is open on 

the way, Sample contamination or no DNA in the tube) the samples may not 

be processed to save your cost.

3) Failure of Reaction 

In the case 2 & 3, our staff may have contacted you via email. 

If you didn’t receive this email even in Spam/Junk folder, please contact us 

immediately.