Sanger DNA Sequencing (Purified PCR Product)
Sanger DNA Sequencing - For unpurified PCR samples add PCR cleanup services along with this service.
PCR product (purified) recommended Concentration 50ng/μℓ
PCR product (non-purified) recommended Concentration 100ng/μℓ
Plasmid recommended Concentration 100ng/μℓ
Require sample Volume : 20~30μℓ
Example: If you send 300bp of PCR product, 30ng/μℓ of a sample require for better results (100bp = 10ng/ μℓ).
It is recommended to check the PCR product concentration through electrophoresis rather than Nano-drop.
To cleanup PCR products please use commercially available PCR purification spin column kits.